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Bioinformatics programs for the design of gRNA and search of off-target cuts.

Journal: International Journal of Molecular Medicine

Article Title: Genome editing: A perspective on the application of CRISPR/Cas9 to study human diseases (Review)

doi: 10.3892/ijmm.2019.4112

Figure Lengend Snippet: Bioinformatics programs for the design of gRNA and search of off-target cuts.

Article Snippet: Identification of the optimal gRNA among various candidates for a given target site, and the localization of potential off-target sites can be supported by different bioinformatics tools ( ); for example: CRISPRdirect , E-CRISPR , WU-CRISPR , CRISPR gRNA design tool ( https://www.atum.bio/eCom-merce/cas9/input ), sgRNA Designer , sgRNA Scorer 2.0 ( , ), CRISPRscan , CRISPR-ERA , CCtop , CRISPOR , Breaking-Cas , CHOPCHOP , CRISP MultiTarget , GT-Scan , ge-CRISPR , CRISPR Design , Cas-Designer , Cas-OFFinder , COSMID , DESKGEN Guide Picker , and CRISPR Genome Analyzer ( ) ( ).

Techniques: CRISPR, Activation Assay, Selection, Biomarker Discovery, Sequencing